
FAST-EM
Biological MicroscopyTomographic Microscopy
Acquire mass and volume information in real time with label-free, quantitative imaging for live cells, assays, tissues and organoids.
GLIM is an upgrade for your existing microscope that enables label-free, quantitative imaging for live cells, assays, tissues and organoids.
The GLIM module is connected to the imaging port of a Phase Contrast microscope, with a camera connected at its output. The microscope separates the illumination into a sample and a reference beam with a phase shear between them that pass through the sample and are collected at the imaging port. The beams pass through the GLIM module where an electro-optical system introduces four accurately controlled phase delays between them. The GLIM camera acquires an intensity image for each phase delay. The intensity images are combined by interference and a recombination algorithm outputs the quantitative OPL map of the entire field of view of the microscope objective. The OPL map is converted to specimen height/volume, dry mass, and refractive index.
GLIM rejects much of the multiple scattering contributions present in an optically thick specimen (e.g. embryos and 3D organoids): the two imaging beams are always equal in power, and suffer equal degradation (that is, the same background noise) because of multiple scattering in the sample such that they interfere with high contrast.
For more on GLIM check out this Nature paper.
GLIM Paper – Nature Communications (2017) https://www.nature.com/articles/s41467-017-00190-7
Phase Imaging with Computational Specificity (PICS) is a new feature of Phi Optics QPI instruments GLIM and SLIM modules that enables various 2D (monolayer) and 3D (organoids) assays to have the accuracy and specificity of regular fluorescence but without the inconveniences associated fluorescent imaging of phototoxicity and photobleaching.
Click here to learn more about PICS.
Details about this new imaging capability was also published in Nature.
Bovine embryo imaged using Phi Optics gradient light interference microscopy GLIM.