Poietis NGB-R

A new, disruptive, 4D bioprinting approach to make complex, functional, living tissues.

Next Generation Bioprinting (NGB) plaform has been developed to overcome current tissue manufacturing methods which are the bottleneck impeding standardisation, scale-up and large-scale market adoption of Tissue-Engineering products.

  • Key Features

    • Computer-Assisted Design. NGB platform integrates a specific « cytocentric », user-friendly CAD software.
    • Automated, robotic bioprinting with easy-to-use bioprinter based on laser-assisted bioprinting.
    • Multimodal traditional extrusion and micro-valve print-head options for complete flexibility in tissue type and complexity.
    • In-line monitoring of the print process combined with IA machine learning algorithms
    • Tissue formation modeling via dedicated software to program tissue self-organization

Next Generation Bioprinting (NGB) plaform has been developed to overcome current tissue manufacturing methods which are the bottleneck impeding standardisation, scale-up and large-scale market adoption of Tissue-Engineering products. It solves critical limitations of existing 3D bioprinting technologies thanks to single-cell resolution and learning-based methods.

Indeed, largely inspired by the principles of the 4.0 Industry, this new platform integrates automation and robotics technologies, coupled with numerous online sensors – including cell microscopy – and Artificial Intelligence processing. In addition, it integrates all bioprinting techniques (laser-assisted bioprinting, bioextrusion, micro-valve bioprinting), a world’s first in the bioprinting market.

  • Designing 3D Biological Structures with CAD

    NGB platform integrates a specific « cytocentric », user-friendly CAD software. This software can be used to design the location and local environment of differe­nt cell types and materials in three-dimensional tissue structures.

    CAD files describe the architecture of biological tissues with the 3D organisation of tissue components (cells and extracellular matrix).

  • Integrated Multi-Modal Bioprinting

    NGB platform integrates a multimodal, easy-to-use bioprinter based on laser-assisted bioprinting capable of 3D-printing tissue components in sterile conditions, with great accuracy and reliability thanks to 6-axis robotic arm and automation. This bioprinter is now the 4th printer version from the beginning of the project in 2005 and the only fully mutlimodal on the market.

  • Laser-Assisted Bioprinting in Action

    Laser-assisted bioprinting makes it possible to print cell by cell according to the following physical mechanism. The focusing of a laser pulse (in blue) on a cartridge (composed of an ink film spread on a glass plate) results in the formation of an ink jet towards a substrate on which cell microdroplets are collected. By controlling the physical conditions of the ejection (energy, viscosity…), the volume of the droplets is controlled precisely (~ picoliter).
    The cell patterns are obtained by rapid scanning of the cartridge by the laser, which results in the formation of 10,000 droplets per second.

  • Accurately Bioprinting Designs

    Bioprinting technologies have been combined with a specific imaging system coupled with AI machine learning algorithms to confirm that what is designed is what is bioprinted.

  • The Evolution of Bioprinted Constructs

    Data generated at all steps of the process are used to model tissue formation. A dedicated software is now under development to program tissue self-organization, which means to anticipate the evolution of the bioprinted construct with time.

  • Making the Most Out of the NGB-R Bioprinter

    All you need to know about the Poietis next generation NGB-R bioprinter:

    • 0.22min –     1. Poietis introduction
    • 2.35min –     2. NGB-R in a nutshell
    • 7.40min –     3. A Cytocentric Approach to Bioprinting
    • 9.05min –     3.1. Printing cells separately & no need for commercial bioinks
    • 16.22min –   3.2. Defining 4D bioprinting & cell patterning
    • 21.00min –   3.3. On-board imaging to take bioprinting farther

    Run time: 26.38min