One of the big challenges in Drug Discovery today is that all current life science tools working at the molecular level either measure static structure or average binding kinetics; the crucial and often very complex mechanical details of the underlying dynamic process are often not revealed. Dynamic Single-Molecule (DSM) analysis reveals these details. This reduces the risk of failure of drugs in costly late-stage clinical trials, thereby decreasing the overall cost of drug development dramatically.
DSM analysis has the unique ability to combine live imaging, manipulation and force-displacement measurement with Ångström precision for the study of DNA-protein interactions and protein conformational changes. This combination gives the crucial dynamic and functional mechanistic information that is complementary to structural data (X-ray crystallography) and ensemble average kinetics (SPR or calorimetry). In pharma, DSM can play a significant role in two stages of the Drug Discovery Process: Target Validation, and Hit-to-Lead.
Applications of DSM in drug discovery
Study protein conformational changes in real-time at the single-molecule level
Study the mechanism behind the biological function of proteins by looking at the conformational changes of protein targets, in real-time and with Ångström resolution
Observe conformational changes of protein targets in the presence of different pharmaceuticals. Find out whether the desired mechanism(s) of action is fulfilled by the drug in a quick, easy and effective manner