An important challenge in imaging in-vitro 3D structures with any optical method is loss of contrast due to multiple scattering. Gradient Light Interference Microscopy (GLIM) rejects much of the multiple scattering contributions and exhibits strong optical sectioning needed for 3D label-free and quantitative imaging of these specimens. Phi Optics GLIM system has been used by various research groups to visualise the 3D time-lapse evolution of optically thick specimens such as organoids, embryos, tissues and small model animals (zebra fish, C. elegans).
Phi Optics implements GLIM technology into a compact add-on module that is compatible with most light microscopes and provides imaging with any magnification from 1X to 100X, dry or immersion objectives. Phi Optics CellVista software suite controls the microscope motorisation to provide 4D (3D time lapse) multi-channel imaging including fluorescence. The same camera is used for GLIM and fluorescence channels for seamless image registration. GLIM’s exceptional sensitivity and SNR can be combined with machine-learning algorithms for digital staining of live specimens: Phase Imaging with Computational Specificity (PICS).
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