Automated live cell imaging: The CX-A

Holotomography Microscopy

A unique walk-away solution for long term live cell imaging. Now on scientists can look at how living cells function and react under various experimental conditions, in parallel and throughout the cell’s lifetime.

  • Key Features

    • Long-term live cell incubation with automation - Heat, humidity, CO2 and O2 control for long term stable timelapse imaging of even the most sensitive cells
    • Non- invasive 3D characterization  - Live cell imaging in physiological conditions without any bleaching or phototoxicity
    • Label-free 4D continuous Observation  - Measurement of cell processes from seconds to weeks
    • Multiplexing, Resolution to below 200nm in X-Y  - High resolution and high sensitivity characterization of multiple cell organelles based on their refractive index.
    • Full 3D scan in under 2 seconds  - Fast imaging for capturing dynamic processes, organelles, single cell and cell populations
    • Multi-well Plate - Designed to work with 96 well plates to multiply and parallelize experimental conditions

Observe living cells from Population to Organelles

Nanolive’s CX-A extends the exploration of living cells from single cells to cell populations without compromising on the highest precision and resolution proper to all Nanolive products.  It enables scientists to investigate macro cellular dynamics like cell health, proliferation, movement and function as well as micro organelle dynamics and interactions e.g. mitochondrial network characterization.

(Multiplex)³

The CX-A is designed to work with 96 well plates to multiply and parallelize experimental conditions, hence, bringing undoubtable significance to each experiment and delivering solid biological insights to researchers (1).

Furthermore, the system is equipped with multiple imaging modalities to correlate and compare physical and chemical information at each time-point (2).

Finally, 3D data sets of every single image at every single time-point are automatically acquired in real-time (3). A fully integrated solution adapted to the most advanced professional needs.

(1).96 well plate overview  (2). Multiple organelle detection at each acquisition
(3). 3D acquisition (depth: 30 μm)

Long-term live cell incubation (Walk-away with automation)

Nanolive’s CX-A automates data acquisition, thus delivering new insights and understanding into biological processes. 

Nanolive’s completely redesigned intuitive user-interface enables first-time users to set-up experiments in just a few minutes and walk away, while the CX-A automatically collects the images. In addition to improving data significance, multiple imaging regimens can be programmed within the same plate allowing users to run different applications in parallel.  A real-time preview allows the user to navigate through the data at any time while offering an overview of the experimental protocol. The 3D dataset output can then be exported with just a few clicks to multiple data formats for analysis.

Unique imaging technique (Image cells from seconds to weeks)

The CX-A can image living cells for a virtually limitless amount of time. Thanks to its unique harmless way of cell preparation and observation, hundreds of images can be collected each hour, transforming endpoint assays to continuous analysis, for days or weeks, while cells remain unperturbed in a physiologically controlled environment. Users can freely define the perfect imaging regimen for their cells without worrying about phototoxicity or photobleaching.

These unique properties of Nanolive’s CX-A system enable users to continuously monitor their precious, most-sensitive cells and to study their finest behaviors without missing anything.

  • Technical Specifications (See what you have been missing)

    • Illumination source: Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2). Epifluorescence: High speed switchable <100 μs, Lifetime >20’000 hours
    • Resolution: 3D Holotomography (HT): x,y: 200 nm; z: 400 nm. Epifluorescence: x,y: ~ 400 nm (depending on channel)
    • Field-of-view (FoV) : Single FoV* / 2×2 FoV / 3×3 FoV *Single FoV: RI: 90x90x30 μm; Fluorescence: 90×90 μm
    • Channels: HT: Up to 8 organelles simultaneously. Epifluorescence: DAPI + FitC  + TritC | FitC + TritC + Cy5 | DAPI + FitC + TritC/Cy5
    • Imaging modalities: Automated: 3D HT | 3D HT + Epifluorescence | 4D HT time-lapse | 4D HT + Epifluorescence time-lapse. 2 Sample holder  Nanolive’s 96 well plates, designed for high precision imaging with optical quality glass bottom and lid
    • Autofocus: High precision label-free autofocus for stable long-term observations in all imaging modalities
    • Incubator stage-top: Tokaihit stage top incubator: CO2 concentration range: 5% – 20% (±0.1%); Humidity: ~ 95%; Sample temperature: 30-40°C (±0.3°C)
    • Camera: USB 3.0 CMOS Sony IMX174 sensor / Quantum Efficiency (typical) 70 % (at 545 nm) / Dark Noise (typical) 6,6 e¯ / Dynamic Range (typical) 73,7 dB
    • Microscope Objective: Dry objective / 60x magnification / NA 0.8
    • Weight  ~30Kg
  • Automated live cell imaging: The CX-A

    For further information and  more Nanolive videos please follow the link below.

    https://nanolive.ch/cx-a/

     

  • Automated microscope gives scientists a look inside live cell populations

    From now on scientists can look at how living cells function and react under various experimental conditions, in parallel and throughout a the cell’s lifetime. With the new 3D microscope unveiled today by EPFL spin-off Nanolive, researchers can observe the details of how cells operate – all the way down to their organelles. What’s more, the microscope is fully automated and doesn’t damage the cells being studied.

  • CX-A webinar

    Did you miss the webinar about the newest automated label-free live cell imaging tool?

    Here is a short preview. Enjoy the 20-minutes long webinar here: https://nanolive.ch/cx-a/#webinar

  • All
  • AFM/SPM/SNOM
  • Automated Mineralogy
  • Biological Microscopy
  • Cell Culture
  • CL
  • CLEM
  • Computed Tomography
  • Confocal Microscopy
  • Diffraction Imaging
  • Digital Microscopy
  • DNA/RNA
  • EBSD
  • EDS
  • Electron Beam Lithography (EBL)
  • Electron Microscopy
  • Fabrication
  • FIB
  • Fluorescent Microscopy
  • Hyperspectral
  • In situ
  • Laser spectroscopy
  • Light Microscopy
  • Live Cell Imaging
  • Micro XRD
  • Micro XRF
  • Microscopy
  • Molecular Biology
  • Multiphoton Microscopy
  • Optical Tweezers
  • Pre-clinical Imaging
  • Protein
  • Protein Crystallography
  • Quantitative Phase Imaging
  • Raman
  • SEM
  • Small Molecule
  • Spectroscopy
  • Super Resolution Microscopy
  • TEM
  • Thermal Probe Lithography
  • Tomographic Microscopy
  • WDS
  • X-ray absorption spectroscopy (XANES/EXAFS)
  • X-ray Imaging
  • X-ray Microscopy
  • XRD
  • XRF