Automated Particle Detection and Analysis for Protein Formulations – Hound

Automatically identify unwanted particles and contaminants in your drug batch


The Hound is the ultimate tool to detect unwanted particles that can potentially contaminate the quality of your drug production run. It can automatically locate, identify and characterise the offending particles within minutes using a combination of:

  • Microscopy
  • Raman spectroscopy
  • LIBS – Laser Induced Breakdown Spectroscopy

What Can Hound Measure

Hound can determine the following:

  • Chemical composition
  • Elemental composition
  • Particle count
  • Particle shape
  • Particle size
  • Particle colour

Simple Operation

Step 1

Simply pour your suspicious sample through a filter round or pipette onto a wet round and drop it into your Hound. The gold-coated surfaces catch everything and eliminate the background noise.

Unchained Labs filter round

Step 2 – Microscopy

With all the particles trapped on the gold surfaces, Hound’s microscope automatically images everything including translucent proteins using bright-field, dark field and fluorescence imaging. Using automated image analysis, Hound scans the entire sample identifying the size and shape of every particle, allowing you to easily detect unwanted particles.

Unchained Labs Hound microscopy

Step 3 – Raman Spectroscopy

Raman spectroscopy identifies all proteins and organic materials by comparing their chemical composition to a customisable database of reference materials. You will know straight away if any particle is a protein or contaminant.

Unchained Labs Hound Raman spectroscopy

Step 4 – LIBS

If other particles are detected such as metallic particle from a fermenter or glass particle vial, Hound is able to determine their composition using LIBS (Laser Induced Breakdown Spectroscopy). By shooting the particle with a laser, a tiny amount of materials is ionised into a plasma which is measured with a spectrometer and analysed against a database and identified.

Unchained Labs LIBS analysis


Raman 785nm + LIBSGoodBestBestBest
Raman 532nm + LIBSBestBestGoodBest
Raman 532nm + 785nmBestBestGoodN/A
Raman 785nmGoodBestGoodN/A
Raman 532nmBestBestGoodN/A
Unchained Labs Hound for automated detection of unwanted particles and contaminants


MicroscopeImaging: 2 MP color camera, 5x, 10x, 20x (DF and BF), 50x objectives
Illumination: Integrated bright-field and dark-field illumination
Particle size range: 0.5 μm – 15 mm
Accuracy: ±5% sizing (magnification dependent), ±15% counting
RamanLaser: Adjustable power, 50x, 0.55 NA, 5.0 mm WD
785 nm: ≤50 mW, 200–2000 cm-1, resolution 5 cm-1
532 nm: ≤20 mW, 200–3200 cm-1, resolution 5 cm-1
Minimum particle size: 0.5 μm
Database: 250 typical contaminants, expandable
LIBSLaser: Safety Class 1, Pulse-LASER w/130 μJ, 3 ns duration, 20 x UV lens
Spectral range: 350–700 nm, resolution 1 nm
Minimum particle size: 3 μm
Database: 50 typical contaminants, expandable
Filter RoundsMaterial: Gold coated polycarbonate (PC) — 0.5 μm min. particle size
Gold coated polytetrafluorethylene (PTFE) — 2 μm min. particle size
pH range: 1–14
Wet RoundsMaterial: Gold or nickel coated
Optical transmission: 255–850 nm
Software21 CFR Part 11 & USP <788>, Method 2, ISO 8871-3:2004 compliance included
EnvironmentTemperature range: 15–30 °C
Humidity: 0–95% relative humidity (non-condensing)
Physical propertiesWeight: 30 kg
Dimensions: 60 cm W x 60 cm D x 80 cm H
Recommended bench space: 180 cm W x 80 cm D x 110 cm H
Electrical 230V/500W
Unchained Labs Hound for automated detection of unwanted particles and contaminants

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