Topics covered

• Introduction to detector technology and the resolution ‘revolution’ brought about by direct detectors
• DirectView overview with single-particle cryoEM results from 120kV LaB₆, benefits of motion correction.
• DE-64 Single-Particle: Latest high resolution results, optimising resolution, noise sources in direct detection sensors, CMOS pixel designs, electron counting and DQE, camera comparison with small particles.
• DE-64 tomography: 8k x 8k cellular tomography examples, continuous tilt tomography and estimated throughput.
• DE-64 MicroED: Latest results, using the DE-64 for MicroED, advantages of the DE-64 for MicroED.

Presenter: Dr. Michael Spilman, Applications Specialist at Direct Electron

Michael received his Ph.D. in Biochemistry and Molecular Genetics under the tutelage of Dr. Terje Dokland at the University of Alabama at Birmingham. Focusing on high throughput methodologies, he went on to work with Dr. Scott Stagg at Florida State University on imaging small biological macromolecules. He is broadly interested in applying novel imaging methods using direct electron detectors to bridge high resolution biological structures with real-time dynamics. At Direct Electron, Dr. Spilman delivers both on- and off-site service and support to new and existing customers, and collaborates with customers on numerous projects including single particle cryoEM, microED, and liquid cell in situ.