Rigaku Reagents for Protein Crystallography
Rigaku supply a range of consumables for protein crystallisation. These products are the perfect partner for your Rigaku Protein Crystallisation x-ray diffraction system and TTP Labtech liquid handling and screen optimisation robots. You can be assured of their suitability as they have been designed by structural biologists for structural biologists.
Rigaku’s Wizard screens, originally developed by Emerald BioSystems, were created to provide a wide array of chemical combinations for researchers in order to give you the highest probability of crystallising your proteins of interest. They have been designed to enhance your workflow with the knowledge that no two proteins are alike, and examining the vast number of variables is time consuming.
Wizard Screen Formats
All Wizard screens have been prepared in a 96 well-block plate formulations, are 1.7 ml each in a 96 deep-well matrix block plate, with the exception of the Wizard pH Buffer screen. Tube sets are 10 ml volumes per tube. All formulations have been prepared with ASTM type-1 water and high-purity chemicals, 0.2 μm filtered in sterile packaging. For specific screen formats, click here.
The Wizard Classic suite of sparse matrix method screens are a highly effective starting point in the screening of biological macromolecules. They have been designed to overcome the exhaustive search for suitable protein crystallisation conditions. They feature different concentrations, components and pH values to help you to determine the most favorable conditions for a particular protein as quickly as possible.
Structural genomics centres worldwide use the Wizard Classic suite as initial crystallisation conditions for proteins, peptides, and protein complexes.
The Wizard Classic range has four variations:
- Wizard 2 has many of the same components as Wizard 1, just diff erent concentrations, pHs and pairings with other components. Most users set up their trays 96 conditions at a time, so Wizard 1 and 2 make a good complement for a 96-well plate
- Wizard 3 and 4 were developed from proven, successful solutions from which others have grown crystals. In the case of Wizard 4, the screen was developed from successful hits that EmeraldBio obtained in-house from SSGCID proteins and these were cross referenced to screens 1, 2, and 3 to ensure that there was no overlap or close similarity in conditions
This screen was developed by researchers from the Joint Center for Structural Genomics (JCSG). It was designed to maximise the coverage of the crystallisation parameter space inclusing buffers, salts, precipitants and pH, while at the same time eliminating redundancy. The buffers give final solution pHs between 4.2 and 10.5.
Precipitants include volatile reagents such as ethanol, high molecular weight polymers such as PEG-8000, and salts such as ammonium sulfate.
Wizard PEG Ion
Formulated for the coarse screening of biological macromolecules such as proteins, nucleic acids, peptides, and combinations thereof, the PEG Ion series matrix combine the crystallising power of polyethylene glycols (PEGs) with salts that are commonly found in crystallisation conditions.
The Wizard Cubic LCP (Lipidic Cubic Phase) Kit™ was designed by Emerald Bio’s membrane
protein crystallographers. It allows researchers to prepare LCP-type crystallisations by hand. Wizard Cubic LCP screen is a random sparse matrix designed for the crystallization of biological macromolecules that are embedded within a lipidic cubic phase LCP host matrix.
It is suitable for low-protein quantities, down to sub-mg amounts, the LCP technique has become the workhorse for crystallisation of 7TM membrane proteins.
The kit contains all the tools required to prepare membrane protein crystallisation experiments using the LCP approach.
Every formulation in this 96-well block plate is compatible with monoolein-based lipidic cubic phases.
The Wizard TIME is a detergent kit consisting of 84 different Formulations. Its value is in identifying those detergent reagents that successfully extract a membrane protein from a membrane preparation.
Membrane proteins typically require detergent solubilisation for purification and crystallisation. The choice of the proper detergent is typically restricted to a low number of readily available detergents and their evaluation requires sequential extraction experiments. FSEC–fluorescence size exclusion chromatography has proven to be a useful tool in characterising the extraction yield and behaviour in detergent solution of fluorescently labelled membrane proteins.
Each detergent formulation consists of a detergent at 2% (w/v) concentration, the stabilising co-detergent cholesterol hemisuccinate and a buffer.
Wizard Precipatant Synergy
The Wizard Preciptant Synergy is designed for protein targets that have failed to yield hits with traditional random sparse matrix crystallisation approaches. It targets all types of soluble protein and protein complexes.
Tests have shown that this screen can triple the number of unique crystals when compared with other screens.
Each unique formulation is made available in three different concentrations, increasing the coverage of crystallisation space. These related formulations are positioned in neighbouring wells or tubes, simplifying the analysis of crystallization behaviour.
These screens use 2 x 96 = 192 formulations in two crystallisation plates for initial protein crystallisation screening and crystallisation optimisation.
The Wizard Cryo™ line of random sparse matrix screens has been designed for scientists who want to avoid the additional step of optimising a cryoprotectant condition.
All Wizard Cryo formulations flash-freeze to a clear amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo-conditions.
Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.
Wizard pH Buffer
The Wizard pH Buffer screen is an optimisation screen that can be applied to your crystallisation hit optimisation. It allows you to simultaneously investigate the effects of pH and buffer composition on crystal growth.
The Wizard pH Buffer matrix is a set of 96 unique buffer solutions encompassing 12 unique buffer systems at 8 pH points, each varying by 0.4 pH units.
96-well block plate formulations consist of 1.0 ml samples in a 96 deep-well matrix block plate. All formulations are prepared with ASTM type-1 water and high-purity chemicals, 0.2 μm filtered in sterile packaging.
Wizard Screens originally developed by Emerald Biosystems.
|Name||Tubes||Deep Well Blocks|
|Classic 1||✔||Data Sheet|
|Classic 2||✔||Data Sheet|
|Classic 3||✔||Data Sheet|
|Classic 4||✔||Data Sheet|
|Classic 1 & 2||✔||Data Sheet|
|Classic 3 & 4||✔||Data Sheet|
|JSCG Core Suite 1||✔||Data Sheet|
|JSCG Core Suite 2||✔||Data Sheet|
|JSCG Core Suite 3||✔||Data Sheet|
|JSCG Core Suite 4||✔||Data Sheet|
|PEG Ion with PEG 1K & 4K||✔||Data Sheet|
|PEG Ion with PEG 8K & 10K||✔||Data Sheet|
|Cubic LCP Kit||✔|
|Cubic LCP Screen||✔||Data Sheet|
|Preciptant Synergy||✔||✔||Data Sheet|
|Cryo 1||✔||✔||Tubes Data Sheet
Deep Well Block Data Sheet
|Cryo 2||✔||✔||Tubes data Sheet|
|pH Buffer||✔||Tubes data Sheet|